Journal: Breast Cancer : Targets and Therapy

Article Title: The role of the chemokine receptor XCR1 in breast cancer cells

doi: 10.2147/BCTT.S126184

Figure Lengend Snippet: Expression of XCR1 in breast cancer cell lines. Notes: Expression of XCR1 mRNA was measured by quantitative RT-PCR ( A ) in 1 normal breast epithelial cells and 9 breast cancer cell lines. XCR1 protein expressions were detected by immunohistochemistry in 10 formalin-fixed, paraffin-embedded breast cancer tissue sections (5 tissue sections are ER+, the others are ER−), the expression of XCR1 protein is associated with ER expression ( B , C ). * p <0.05, ** p <0.01. Abbreviations: ER, estrogen receptor; RT-PCR, real-time polymerase chain reaction.

Article Snippet: Human breast cancer cell lines, MDA-MB-231, MDA-MB-468, ZR-75-30, T474D, MCF-7, ZR-75-30, and SK-BR-3, and one normal breast epithelial cell line, namely MCF-10A, were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemistry, Formalin-fixed Paraffin-Embedded, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

Journal: Oncotarget

Article Title: A high-throughput method to detect RNA profiling by integration of RT-MLPA with next generation sequencing technology

doi: 10.18632/oncotarget.17551

Figure Lengend Snippet: The RNA levels of 16 genes, including Ki67, STK15, Survivin, CCNB1, MYBL2, GRB7, HER2, MMP11, CTSL2, ER, PGR, BCL2, SCUBE2, GSTM1, CD68, BAG1 in FFPE sections were normalized to the average Ct value of 5 reference genes (ACTB, GAPDH, RPLPO, GUSB, TFRC).

Article Snippet: RNA was extracted from FFPE breast cancer tissues (QIAGEN RNeasy FFPE Kit) and was reverse-transcribed with random Biotin-(dN) 6 primers.

Techniques: